Plasmid

Part:BBa_K5315003:Design

Designed by: Shiori Kajikawa   Group: iGEM24_Grand-Tokyo   (2024-10-02)


Shuttle vector for conjugational transfer [B. subtilis]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5144
    Illegal XbaI site found at 7357
    Illegal XbaI site found at 7388
    Illegal SpeI site found at 6248
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 6248
    Illegal NotI site found at 535
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1541
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5144
    Illegal XbaI site found at 7357
    Illegal XbaI site found at 7388
    Illegal SpeI site found at 6248
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5144
    Illegal XbaI site found at 7357
    Illegal XbaI site found at 7388
    Illegal SpeI site found at 6248
    Illegal NgoMIV site found at 669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6200
    Illegal SapI site found at 673
    Illegal SapI site found at 3778
    Illegal SapI site found at 6769


Design Notes

The objective gene, aprE, was cloned in place of RFP (between NotI).


Source

This part originates from chimera of bacteria and phage DNAs.


References